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Resistance analysis of eIF(iso)4E -mutated ( Bra035393 ) plants challenged with TuMV by <t>ELISA</t> analysis 21 days post inoculation. Analysis of TuMV coat protein accumulation in inoculated and systemic leaves of single (black bar) and double (gray bar) indel pattern #6 and #7 T 1 edited plants by <t>DAS-ELISA.</t> The mutation patterns were described in . Error bars indicate the mean values of replicates ± SD.
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double-antibody sandwich enzyme-linked immunosorbent assay (das-elisa)  (Agdia Inc)
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Resistance analysis of eIF(iso)4E -mutated ( Bra035393 ) plants challenged with TuMV by <t>ELISA</t> analysis 21 days post inoculation. Analysis of TuMV coat protein accumulation in inoculated and systemic leaves of single (black bar) and double (gray bar) indel pattern #6 and #7 T 1 edited plants by <t>DAS-ELISA.</t> The mutation patterns were described in . Error bars indicate the mean values of replicates ± SD.
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Resistance analysis of eIF(iso)4E -mutated ( Bra035393 ) plants challenged with TuMV by ELISA analysis 21 days post inoculation. Analysis of TuMV coat protein accumulation in inoculated and systemic leaves of single (black bar) and double (gray bar) indel pattern #6 and #7 T 1 edited plants by DAS-ELISA. The mutation patterns were described in . Error bars indicate the mean values of replicates ± SD.

Journal: Horticulture Research

Article Title: CRISPR/Cas9-mediated gene editing to confer turnip mosaic virus (TuMV) resistance in Chinese cabbage ( Brassica rapa )

doi: 10.1093/hr/uhad078

Figure Lengend Snippet: Resistance analysis of eIF(iso)4E -mutated ( Bra035393 ) plants challenged with TuMV by ELISA analysis 21 days post inoculation. Analysis of TuMV coat protein accumulation in inoculated and systemic leaves of single (black bar) and double (gray bar) indel pattern #6 and #7 T 1 edited plants by DAS-ELISA. The mutation patterns were described in . Error bars indicate the mean values of replicates ± SD.

Article Snippet: The symptomatic leaves showing typical TuMV symptoms were harvested four weeks after inoculation and double checked with a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) test kit (Agdia Inc., Elkhart, IN, USA) for the virus presence.

Techniques: Enzyme-linked Immunosorbent Assay, Mutagenesis